Quantitative RT-PCR for RAS Components

Total RNA was extracted from CD34⁺ cells and quantitative RT-PCR was performed as described previously^{14 (link)}. FAM-labeled primers (Applied Biosystems, Foster City, CA) were used: ACE (Hs01104600_m1), ACE2 (Hs01085333_m1), Mas (Hs00267157_s1), AT2R (Hs00169126_m1). All samples were run in duplicate and normalized to 18s (Hs99999901_s1) cDNA.

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Cole-Jeffrey C.T., Pepine C.J., Katovich M.J., Grant M.B., Raizada M.K, & Hazra S. (2018). Beneficial effects of Angiotensin-(1-7) on CD34+ cells from heart failure patients. Journal of cardiovascular pharmacology, 71(3), 155-159.

Publication 2017

FAM-labeled primers

Ace2 Cdna Primers Rt pcr

Corresponding Organization :

Other organizations : University of Florida, University of Alabama at Birmingham

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression levels of ACE (Hs01104600_m1), ACE2 (Hs01085333_m1), Mas (Hs00267157_s1), and AT2R (Hs00169126_m1) genes

control variables

18s (Hs99999901_s1) cDNA used for normalization of gene expression

controls

Positive control: None mentioned
Negative control: None mentioned

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