RP-LC-MS/MS for Ceramide Profiling

The RP-LC-MS/MS was applied to obtain the CER profiles. The same Agilent UPLC-ESI-QTOF-MS system (Agilent 1290; Agilent 6540; Agilent Technologies, Santa Clara, CA, USA) was applied for analysis. Chromatographic separation of CERs was performed on RP-C18 column (Acquity BEH Shield 2.1 × 100 mm; 1.7 μm; Waters, Milford, MA, USA) in gradient elution with the mixture of water (20 mM ammonium formate, pH 5) and methanol. The QTOF mass spectrometer was operated in positive (electrospray voltage 3.5 kV) ion mode with a capillary temperature of 300 °C and a sheath gas flow of 8 L/min, as previously described in details [27 (link)].

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Łuczaj W., Domingues M.D., Domingues P, & Skrzydlewska E. (2020). Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol. Antioxidants, 9(12), 1178.

Publication 2020

UPLC-ESI-QTOF-MS system Agilent 1290 Agilent 6540 Acquity BEH Shield 2.1 × 100 mm; 1.7 μm

Ammonium formate Capillary Chromatographic Methanol Ms ms

Corresponding Organization : Medical University of Białystok

Other organizations : University of Aveiro

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Variable analysis

independent variables

Chromatographic separation of CERs was performed on RP-C18 column (Acquity BEH Shield 2.1 × 100 mm; 1.7 μm; Waters, Milford, MA, USA) in gradient elution with the mixture of water (20 mM ammonium formate, pH 5) and methanol.

dependent variables

The RP-LC-MS/MS was applied to obtain the CER profiles.

control variables

The same Agilent UPLC-ESI-QTOF-MS system (Agilent 1290; Agilent 6540; Agilent Technologies, Santa Clara, CA, USA) was applied for analysis.
The QTOF mass spectrometer was operated in positive (electrospray voltage 3.5 kV) ion mode with a capillary temperature of 300 °C and a sheath gas flow of 8 L/min, as previously described in details [27 (link)].

controls

No positive or negative controls were explicitly mentioned in the provided information.

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