After phage purification, samples were subjected to negative staining transmission electron microscopy (TEM) as described previously by [43 (link)]. After application of 5 μl of sample to glow-discharged and carbon-coated copper grids, the samples were blotted on filter paper and washed twice with double-distilled water. After this, they were negatively stained with 2% uranyl acetate for 20 seconds, blotted again and left for air-drying.
For TEM, a Zeiss EM912 with an integrated OMEGA-filter (Zeiss) and a 2 k × 2 k CCD camera (TRS) was used. The microscope was operated at 80 kV in the zero-loss mode.
For TEM, a Zeiss EM912 with an integrated OMEGA-filter (Zeiss) and a 2 k × 2 k CCD camera (TRS) was used. The microscope was operated at 80 kV in the zero-loss mode.
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