Pyroptotic cell death was examined according to a previously published protocol (37 (link)). Briefly, the cultured BMMSCs were stained with caspase-1 antibody (D-3), FITC (sc-392736; Santa Cruz Biotechnology), and propidium iodide (PI) for 1 h and fixed with 4% paraformaldehyde on ice for 10 min. Following washing with Hanks’ balanced salt solution (Sigma-Aldrich), the cells were analyzed with a BD Accuri C6 Plus cell analyzer (BD Biosciences).
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Pyroptotic Cell Death Quantification
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Antibody
Caspase 1
Cell
Fitc
Hanks balanced salt solution
Paraformaldehyde
Propidium iodide
Pyroptotic cell death
Corresponding Organization : University of Louisville
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Variable analysis
independent variables
- Caspase-1 antibody (D-3), FITC (sc-392736; Santa Cruz Biotechnology)
- Pyroptotic cell death
- Cultured BMMSCs
- Propidium iodide (PI)
- 4% paraformaldehyde
- Hanks' balanced salt solution (Sigma-Aldrich)
- BD Accuri C6 Plus cell analyzer (BD Biosciences)
- No positive or negative controls were explicitly mentioned in the provided information.
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