Culturing Prostate and Liver Cancer Cell Lines

Two prostate cancer-derived cell lines (LNCaP and DU145) and one hepatocellular carcinoma-derived cell line (SNU-387), as well as a non-tumor prostate (PNT2) cell line and hepatoblastoma cell line (Hep-G2) were used in this study. All cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA), except the PNT2 cells, which were generously supplied by Dr. J. De Bono (London, UK). Cells were cultured according to manufacturer instructions as previously described [82 (link),103 (link)]. Briefly, LNCaP, DU-145, PNT2, and SNU-387 cells were cultured on RPMI-1640 (Lonza, Madrid, Spain) supplemented with 10% fetal bovine serum (FBS; Merck KGaA, Damstadt, Germany), 1% L-glutamine (Thermo Fisher Scientific, Madrid, Spain), and 0.2% gentamicin-amphotericin B (Thermo Fisher Scientific), whereas Hep-G2 cells were cultured on MEM (Thermo Fisher Scientific) supplemented with 10% FBS, 1% pyruvate (Thermo Fisher Scientific), and 0.2% gentamicin-amphotericin B at 37 °C in a humidified 5% CO₂ atmosphere. Cell lines were validated by analysis of short tandem repeats (STRs) sequences using GenePrint 10 System (Promega, Barcelona, Spain) and checked for mycoplasma contamination by PCR as previously reported [104 (link)].