Technical grade chlorpyrifos (96% purity) was obtained from Dow AgroSciences, USA. All other chemicals and solvents used were analytical grade.
Activated sludge samples were collected as inoculum from a pesticide-manufacturing wastewater treatment system and chlorpyrifos-degrading fungus Cladosporium cladosporioides Hu-01 was screened and isolated [16] (link). This strain was deposited in China Center for Type Culture Collection under the collection No. CCTCC M 20711. The constant-temperature culture method was referred to Gao et al. [22] and the conditions were inoculum amount 0.1% (wet fungi), 28°C and 150 rpm on a rotary shaker for 5 days. Previously, the degradation of chlorpyrifos by strain Hu-01 was determined. 92.7% of chlorpyrifos at the initial concentration of 25 mg·L−1 was degraded in half hour, which revealed that strain Hu-01 can significantly reduce chlorpyrifos residue.
Activated sludge samples were collected as inoculum from a pesticide-manufacturing wastewater treatment system and chlorpyrifos-degrading fungus Cladosporium cladosporioides Hu-01 was screened and isolated [16] (link). This strain was deposited in China Center for Type Culture Collection under the collection No. CCTCC M 20711. The constant-temperature culture method was referred to Gao et al. [22] and the conditions were inoculum amount 0.1% (wet fungi), 28°C and 150 rpm on a rotary shaker for 5 days. Previously, the degradation of chlorpyrifos by strain Hu-01 was determined. 92.7% of chlorpyrifos at the initial concentration of 25 mg·L−1 was degraded in half hour, which revealed that strain Hu-01 can significantly reduce chlorpyrifos residue.
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