Cytokine Detection in Cell Culture

Concentrations of IL-33, IL-9, and IFN-γ in culture supernatants were detected by ELISAs as previously described (12 (link)). IL-33 capture/detection Abs were purchased from R&D Systems. Recombinant mouse IL-33 (aa109–266) (ELISA standard) was purchased from R&D Systems. Capture/detection Abs for IL-9 and IFN-γ were purchased from BD Biosciences. Recombinant mouse IL-9 and IFN-γ used as the standards in ELISAs were purchased from R&D Systems and BD Biosciences, respectively. Avidin-HRP was purchased from BioLegend.

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Chen J., Zhao Y., Jiang Y., Gao S., Wang Y., Wang D., Wang A., Yi H., Gu R., Yi Q, & Wang S. (2018). Interleukin-33 Contributes to the Induction of Th9 Cells and Antitumor Efficacy by Dectin-1-Activated Dendritic Cells. Frontiers in Immunology, 9, 1787.

Publication 2018

Recombinant mouse IL-9 Avidin-HRP

Avidin hrp Elisas Ifn γ Il 33 Mouse

Corresponding Organization : First Hospital of Jilin University

Other organizations : Union Hospital, Cleveland Clinic Lerner College of Medicine

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Variable analysis

independent variables

Concentrations of IL-33, IL-9, and IFN-γ

dependent variables

Concentrations of IL-33, IL-9, and IFN-γ in culture supernatants

control variables

Recombinant mouse IL-33 (aa109–266) (ELISA standard) purchased from R&D Systems
Recombinant mouse IL-9 and IFN-γ used as the standards in ELISAs purchased from R&D Systems and BD Biosciences, respectively
Avidin-HRP purchased from BioLegend

controls

Positive control: Recombinant mouse IL-33 (aa109–266), recombinant mouse IL-9, and recombinant mouse IFN-γ used as ELISA standards
Negative control: Not explicitly mentioned

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