Metagenome Library Preparation and Sequencing

Qualifying DNA samples were randomly interrupted using a Covaris ultrasonic crusher and produced approximately 150 bp libraries (Bowers et al., 2015 ). The whole libraries were prepared using the steps of end repair with a 3′ A tail, ligation of adapters and purification (Bentley et al., 2008 (link)). After that, library quality was assessed and sequenced with 150 bp paired-end reads based on the previous methods (Tringe and Rubin, 2005 (link); Fu et al., 2020 (link)). Finally, we obtained an average of 540 million metagenomic-jointed reads (12 Gb) per sample from the sequencing platform (Edgar, 2004 (link)).