The plants were processed with the following treatments in the experiment to analyze the gene expression patterns in soybean plants (G. max) [86 (link)]. In order to determine the dose-dependent expression patterns, the seedings were cultivated in a solution with 0, 50, 100, 150, and 200 mM NaCl for 8 h. The plant materials were collected after 0, 2, 4, 8, 12, and 24 h in the 200 mM NaCl treatment to determine the time-dependent expression patterns. To determine organ-specific expression, the plant organs stored in the −80 °C refrigerator described in Section 4.1 were used for qPCR identification [31 (link)].
The total RNA was extracted from the plant samples using the RNA-easy Isolation Reagent (Vazyme, Nanjing, China) according to the manufacturer’s instructions and then reverse transcribed into cDNA using PrimeScript RT kit (Takara, Shiga, Japan). The gene expression level was evaluated by qPCR using the 2−ΔΔct method using real-time PCR on a CFX96™ Touch Real-Time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Premix ExTaq™ II Mix (TaKaRa, Shiga, Japan) [86 (link)]. GmACTIN6 (GeneBnak Accession: AAK285830.1) was used as an internal control for three technical replicates. All primers were designed using the NCBI Primer tool (http://www.ncbi.nlm.nih.gov/ , accessed on 4 February 2022) and are shown in Table S2 .
The total RNA was extracted from the plant samples using the RNA-easy Isolation Reagent (Vazyme, Nanjing, China) according to the manufacturer’s instructions and then reverse transcribed into cDNA using PrimeScript RT kit (Takara, Shiga, Japan). The gene expression level was evaluated by qPCR using the 2−ΔΔct method using real-time PCR on a CFX96™ Touch Real-Time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Premix ExTaq™ II Mix (TaKaRa, Shiga, Japan) [86 (link)]. GmACTIN6 (GeneBnak Accession: AAK285830.1) was used as an internal control for three technical replicates. All primers were designed using the NCBI Primer tool (
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