HeLa cells were treated with 15 µM APE1 inhibitor III (APE1inhIII) (Merck Millipore, Burlingtion, MA, USA) or the solvent DMSO (Merck Millipore, Burlingtion, MA, USA) for 5 h at 37 °C [28 (link)] before mitochondrial replication imaging protocol (mREP) or total DNA isolation was performed. For scavenging of ROS, HeLa cells were treated for 24 h with either 100 µM mitoTempo (Sigma-Aldrich/Merck, St. Louis, MO, USA) [37 (link)] or N-acetyl-L-cysteine (NAC) (Sigma-Aldrich/Merck, St. Louis, MO, USA) [29 (link),38 (link),39 (link),40 (link)] or water as solvent. To block mitochondrial import of proteins, HeLa cells were treated for 48 h with 10 µM mitoBlock-6 (Focus Biomolecules, Plymouth Meeting, PA, USA). To slow down mitochondrial DNA replication, the HeLa cells were treated for 24 h with 10 µM 2′,3′-Dideoxycytidine (ddC) (Sigma-Aldrich/Merck, St. Louis, MO, USA) [41 (link)].
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