Cell Viability Assay in Breast Cancer

Cell viability assay was conducted as previously described [40 (link)]. Breast cancer control, GPR141 overexpressed and knockdown cells were seeded in 96-well cell culture plates (Corning, Pune, India) at 3 × 10³ cells/well density. After 48 h incubation, 10 μL of MTT (MP Biomedical) (5 mg/mL in PBS) was added to each well and incubated at 37°C, 5% CO2 atmospheric condition for another 3 h. Then the medium was removed, and 100 μL of DMSO was added to dissolve the formed formazan crystals. The solubilized crystals were quantified by scanning the plates at 570 nm using Varioskan™ Flash Multimode Reader (Thermo Fisher Scientific, Bangalore, India).