Versatile Fluorescent Protein Expression Plasmids

pCAG-GFP (Addgene plasmid 11150) (Matsuda and Cepko, 2004 (link)). pCAG-YFP (Addgene plasmid 11180) (Matsuda and Cepko, 2004 (link)). pCAG-DsRed (Addgene plasmid 11151) (Matsuda and Cepko, 2004 (link)). pRho-GFP-IRES-AP (referred to as Rho-GFP) (Emerson and Cepko, 2011 (link)). pCAG-nlacZ (Cepko lab, Harvard Medical School) pCAGEN (Addgene plasmid 11160) (Matsuda and Cepko, 2004 (link)). pCALNL-DsRed (Addgene plasmid 13769) (Matsuda and Cepko, 2004 (link)). pCAFNF-DsRed (Addgene plasmid 13771). (Matsuda and Cepko, 2004 (link)). pCALNL-luc2 (Tang et al., 2015 (link)). pRL-TK (#E2241; Promega, Madison, WI).

Free full text: Click here

Tang J.C., Drokhlyansky E., Etemad B., Rudolph S., Guo B., Wang S., Ellis E.G., Li J.Z, & Cepko C.L. (2016). Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodies. eLife, 5, e15312.

Publication 2016

pCAG-GFP pCAG-YFP pCAG-DsRed pCAGEN pCALNL-DsRed pRL-TK

Ires Plasmid Promega

Corresponding Organization :

Other organizations : Howard Hughes Medical Institute, Harvard University, Brigham and Women's Hospital

Top 5 similar protocols

Variable analysis

independent variables

PCAG-GFP (Addgene plasmid 11150) (Matsuda and Cepko, 2004)
PCAG-YFP (Addgene plasmid 11180) (Matsuda and Cepko, 2004)
PCAG-DsRed (Addgene plasmid 11151) (Matsuda and Cepko, 2004)
PRho-GFP-IRES-AP (referred to as Rho-GFP) (Emerson and Cepko, 2011)
PCAG-nlacZ (Cepko lab, Harvard Medical School)
PCAGEN (Addgene plasmid 11160) (Matsuda and Cepko, 2004)
PCALNL-DsRed (Addgene plasmid 13769) (Matsuda and Cepko, 2004)
PCAFNF-DsRed (Addgene plasmid 13771) (Matsuda and Cepko, 2004)
PCALNL-luc2 (Tang et al., 2015)
PRL-TK (#E2241; Promega, Madison, WI)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!