Quantification of PCV2 in Serum

Total nucleic acids were extracted from serum samples using a MagNA Pure LC total nucleic acid isolation kit (Roche Diagnostics GmbH, Mannheim, Germany) operated on a MagNA Pure LC 2.0 automatic nucleic acid extraction instrument (Roche Diagnostics GmbH, Mannheim, Germany), according to the manufacturer’s instructions. To quantify the PCV2 load in serum samples, quantitative real-time PCR assay was tested and performed with a LightCycler^® 96 system (Roche Applied Science, Basel, Switzerland) as described Tsai et al. [18 (link)].

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Lin C.N., Ke N.J, & Chiou M.T. (2020). Cross-Sectional Study on the Sero- and Viral Dynamics of Porcine Circovirus Type 2 in the Field. Vaccines, 8(2), 339.

Publication 2020

MagNA Pure LC MagNA Pure LC 2.0 LightCycler® 96 system

Assay Diagnostics Isolation Nucleic acid Quantitative real time pcr Serum

Corresponding Organization : National Pingtung University of Science and Technology

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Variable analysis

independent variables

Extraction method: MagNA Pure LC total nucleic acid isolation kit (Roche Diagnostics GmbH, Mannheim, Germany)
Extraction instrument: MagNA Pure LC 2.0 automatic nucleic acid extraction instrument (Roche Diagnostics GmbH, Mannheim, Germany)
QPCR assay: Quantitative real-time PCR assay
QPCR instrument: LightCycler® 96 system (Roche Applied Science, Basel, Switzerland)

dependent variables

PCV2 load in serum samples

control variables

Manufacturer's instructions for MagNA Pure LC total nucleic acid isolation kit
Tsai et al. [18] qPCR assay protocol

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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