Example 6
Both RGA 1 and RGA 2 gene functions can be validated with different methods well known in the art. Genetic transformation of a susceptible wheat cultivar overexpressing RGA 1 or RGA 2 under different promoters can be obtained and tested for their ability to confer OWBM resistance in glass-house conditions or in the field.
Validation can also be achieved by mutagenesis with methods known from skilled person in the art, with for example, EMS treatment. The validation consists of obtaining several independent “loss-of-resistance” mutants derived from the EMS treatment of a resistant wheat cultivar and further identifying mutations within the candidate gene; thus confirming the resistance function of the gene. For example, such method is described by Periyannan et al. (2013) used to identify the wheat stem rust resistance gene Sr33.
