Genetic Mouse Models for Developmental Studies

All mice were housed and cared for according to MSSM and IACUC approved protocols. Ezh1^deleted and Ezh2^flox mice were previously reported [45 (link)]. EED^flox mice were provided by Weipeng Mu and Terry Magnuson [70 (link)]. Tcf/Lef:H2B-GFP mice were provided by Anna-Katerina Hadjantonakis [36 (link)]. Gli1^LacZ, Ctnnb1^flox, Smo^flox, Shh^EGFPCre, Atoh1-GFP, R26-rtTA, and Krt14-Cre mice were obtained from The Jackson Laboratory. Wild type C57BL/6 mice were obtained from Charles River Laboratories. Mice were genotyped by PCR using DNA extracted from tail skin. BrdU was administered as previously reported [51 (link)]. Briefly, BrdU was administered (50μg BrdU per 1g mouse weight) to mice or pregnant females 3–5 h before sacrificing [45 (link)].