Phenotypic and Genotypic Detection of KPC Carbapenemase

Phenotypic detection of KPC carbapenemase was performed using the KPC/MBL/OXA-48 Confirm Kit (Rosco Diagnostica, Taastrup, Denmark) and the immunochromatography test O.K.N.V.I. RESIST-5 (CORIS BioConcept, Gembloux, Belgium). Susceptibility testing by double-disk synergy test (DDST) and ESBL/AmpC Screen Kit tests (Rosco Diagnostica) were also performed. KPC carbapenemase genes were detected by the Eazyplex-Superbug-CRE system (Amplex-Biosystems, Germany) and the Xpert Carba-R assay (Cepheid, Sunnyvale, USA) and then confirmed by PCR and Sanger sequencing.

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Castillo-Polo J.A., Hernández-García M., Maruri-Aransolo A., de la Vega C., Ruiz-Garbajosa P, & Cantón R. (2024). Evolution of ceftazidime-avibactam and cefiderocol resistance in ST131-H30R1-Escherichia coli isolates with KPC-3 mutants and application of FTIR biotyping. Microbiology Spectrum, 12(4), e02776-23.

Publication 2024

. RESIST-5 Xpert Carba-R assay

Corresponding Organization :

Other organizations : Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramón y Cajal, Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red

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Variable analysis

independent variables

Phenotypic detection of KPC carbapenemase using the KPC/MBL/OXA-48 Confirm Kit and the immunochromatography test O.K.N.V.I. RESIST-5
Susceptibility testing by double-disk synergy test (DDST) and ESBL/AmpC Screen Kit tests
Molecular detection of KPC carbapenemase genes using the Eazyplex-Superbug-CRE system and the Xpert Carba-R assay

dependent variables

Presence or absence of KPC carbapenemase
Antibiotic susceptibility profiles
Presence or absence of KPC carbapenemase genes

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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