DIANA-TarBase v8.0 caters more than one million entries, corresponding to the largest compilation of experimentally supported miRNA targets. This collection of miRNA–gene interactions has been derived from experiments employing >33 distinct low-yield and high-throughput techniques, spanning 85 tissues, 516 cell types and ∼451 experimental conditions from 18 species (Figure 1A). Approximately 1200 publications were manually curated and >350 high-throughput datasets have been analyzed. The new database version incorporates an assortment of positive and negative direct miRNA interactions. It comprises >10 000 interactions derived from specific techniques. Approximately 5100 of these miRNA targets are verified by reporter gene assays, extracted from ∼950 publications, providing a 1.6-fold increase compared to relevant entries in TarBase v7.0. More than 14 000 direct miRNA–mRNA chimeric fragments defined from CLASH and CLEAR-CLIP experiments, as well as from a previous meta-analysis of published AGO-CLIP datasets (25 (link)), have been integrated to the repository. Approximately 90 000 new entries were generated from the analysis of additional AGO CLIP-seq libraries from three studies. More than 233 000 interactions have been extracted from miRNA-specific transfection/knockdown microarray, RPF-seq, RIP-seq and RNA-seq experiments which were performed in 28 tissues and 82 cell types under 206 experimental conditions. Updated entries derived from the aforementioned methodologies are summarized in Figure 1B.