BXPC-3, PANC-1 and SW1990 (all from Shanghai Genechem Co., Ltd., Shanghai, China) pancreatic cancer cell lines were cultured at 37°C in an atmosphere containing 5% CO2 in RMPI-1640 (Hyclone; Thermo Fisher Scientific, Inc.) or DMEM, supplemented with 10% FBS. Subsequently, CD44+CD24+ cells were sorted from the three cell lines using flow cytometry as previously described (19 (link),20 (link)).
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Isolation of Pancreatic CSCs
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Corresponding Organization :
Other organizations : First Affiliated Hospital of Xinjiang Medical University, Xinjiang Medical University
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Variable analysis
independent variables
- Cell lines (BXPC-3, PANC-1, and SW1990)
- CD44+CD24+ cells
- Culture conditions (37°C, 5% CO2, RPMI-1640 or DMEM with 10% FBS)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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