The bacterial strains phenotypically studied and genome sequenced in this study were drawn from the Burkholderia Table 1 ). A complete list of the 283 isolates and their genomes analysed within the study is provided in Table S1, available in the online version of this article. The isolates studied were recovered from a range of sources, including CF, chronic granulomatous disease (CGD), non-CF clinical infections (NON-CF), the natural environment (ENV) and the healthcare environment (ENVH). Stock cultures were stored at −80 °C in cryogenic vials by resuspension of fresh growth in tryptic soya broth (TSB; Oxoid) containing 8 % (v/v) dimethyl sulfoxide (Sigma-Aldrich). Culture purity was determined by plating frozen stocks onto tryptic soy agar (TSA) (Oxoid) and incubating plates for 24–48 h at 37 °C. Overnight cultures were made by taking a swab from a fresh TSA plate and transferring into 3 ml of TSB. Cultures were grown for 18–20 h at 37 °C using continuous shaking on a rotating platform set to 150 r.p.m.
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