Illumina Library Preparation Protocol

These included 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and 1-methylimidazole (Im) (Macklin Biochemical, Shanghai, China); 20× SSC buffer (GenClone Biotechnology, Beijing, China); 2× Phanta Max Master Mix, VAHTS Universal DNA Library Prep Kit for Illumina V3/ND607, VAHTS HiFi Amplification Mix/N616, VAHTS DNA Adapters for Illumina/N805, VAHTS DNA Clean Beads, Taq DNA polymerase, dNTP Mix (Vazyme Biotech, Nanjing, China); 10,000× SYBR Green I, Betaine and Bovine Serum Albumin (Solarbio, Beijing, China); formamide (Yuanye, Shanghai, China); OP AminoSlideTM (CapitalBio Technology, Chengdu, China); Qubit 1X dsDNA HS Kit, Qubit™ 4 Fluorometer, ProFlex™ 96-Well PCR System (Thermo Fisher Scientific, Waltham, MA, USA); and Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA, USA).