PEDV Pintung 52 Passage 7 Viral Stock

The highly virulent viral stock of PEDV Pintung 52 passage 7 (PEDVPT-P7) was derived from PEDVPT-P5 (GenBank accession no. KY929405) as described in previous studies [19 (link),20 (link),21 (link)]. Vero C1008 cells (American Type Culture Collection no. CRL-1586) were used for viral preparation and the neutralizing assay. The culture medium was Dulbecco’s modified Eagle medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (GE Healthcare, Uppsala, Sweden), 250 ng/mL amphotericin B, 100 U/mL penicillin, and 100 µg/mL streptomycin. The viral titer of PEDVPT-P7 was 1.78 × 10⁵ TCID₅₀/mL, as determined using the endpoint titration assay with a ten-fold serial dilution in triplicates.