The promoter site of LPCAT1 and a mutated form (CGCCCAGGC) of this site were cloned into a dual-luciferase reporter vector (Promega Corporation). The reporter vector was co-transfected along with oe-FOXA1 or oe-NC into MDA-MB-231 cells using FuGENE® transfection reagents (Promega Corporation). At 48 h post-transfection, the luciferase activity was assessed using the Dual-luciferase Reporter Assay System (Promega Corporation), according to the manufacturer's protocol, and normalized to Renilla luciferase activity.
Protocol detail
Evaluating LPCAT1 Promoter Activity
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Corresponding Organization :
Other organizations : Shanxi Provincial Cancer Hospital
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Variable analysis
independent variables
- Oe-FOXA1
- Oe-NC
- Luciferase activity
- Renilla luciferase activity
- LPCAT1 promoter site
- Mutated LPCAT1 promoter site (CGCCCAGGC)
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