In this study, we used the following chemicals: tetrahydrofuran (THF) (186562, Sigma-Aldrich), dibenzyl ether (DBE) (108014, Sigma-Aldrich), -tetrakis (2-hydroxypropyl) ethylenediamine (EDTP) (T0781, Tokyo Chemical Industry Co., Ltd.), DL-alpha-tocopherol (vitamin E) (A17039, Alfa Aesar), and triethylamine (80134318, Sinopharm Chemical Reagent Co. Ltd.).
For dehydration solutions, THF was mixed with according to the following concentration: 50% (v/v), 70% (v/v), 80% (v/v), and 100% (v/v). triethylamine was added to adjust the pH to about 9.0. For clearing agents, the eFDISCO+ was prepared by adding 0.5% (w/v) EDTP to the DBE, and the vFDISCO+ was prepared by adding 0.5% (v/v) vitamin E to the DBE.
The column absorption chromatography with basic activated aluminum oxide (20001861, Sinopharm Chemical Reagent Co. Ltd.) was used to remove the peroxides produced in THF and DBE, as described in the literature.19 (link),20 (link)
For dehydration solutions, THF was mixed with according to the following concentration: 50% (v/v), 70% (v/v), 80% (v/v), and 100% (v/v). triethylamine was added to adjust the pH to about 9.0. For clearing agents, the eFDISCO+ was prepared by adding 0.5% (w/v) EDTP to the DBE, and the vFDISCO+ was prepared by adding 0.5% (v/v) vitamin E to the DBE.
The column absorption chromatography with basic activated aluminum oxide (20001861, Sinopharm Chemical Reagent Co. Ltd.) was used to remove the peroxides produced in THF and DBE, as described in the literature.19 (link),20 (link)
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