Genetically Engineered Mouse Models for NF1 and LATS Studies

The Nf1 knockout (8 (link)), Nf1 flox (9 (link)), Hoxb7-Cre (47 (link)), Lats1 flox (48 (link)), Lats2 flox (48 (link)) ROSA26-YFP (49 (link)), ROSA26-lacZ and athymic nude mice (Foxn1^−/−) are available from Jackson Laboratory (Bar Harbor, ME). Genotyping was performed by PCR as previously reported (8 (link), 9 (link), 47 (link)–49 (link)). All mice were housed in the Animal Care Facility at The University of Texas Southwestern Medical Center (Dallas, TX), and all procedures were approved by Institutional Animal Care and Use Committee at University of Texas Southwestern Medical Center and conformed to NIH guidelines. Human subjects and cNF sample collection and use were approved by Institutional Review Board at University of Texas Southwestern Medical Center and conformed to NIH guidelines. Written informed consent was obtained from patients.

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Chen Z., Mo J., Brosseau J.P., Shipman T., Wang Y., Liao C.P., Cooper J.M., Allaway R.J., Gosline S.J., Guinney J., Carroll T.J, & Le L.Q. (2018). Spatiotemporal loss of NF1 in Schwann cell lineage leads to different types of cutaneous neurofibroma susceptible to modification by the Hippo pathway. Cancer discovery, 9(1), 114-129.

Publication 2018

Nf1 flox Hoxb7-Cre Lats1 flox Lats2 flox ROSA26-YFP ROSA26-lacZ athymic nude mice (Foxn1−/−)

Animal Athymic nude mice Foxn1 Institutional animal care and use committee Institutional review board Lacz Lats1 Lats2 Mice Patients Sample collection

Corresponding Organization : The University of Texas Southwestern Medical Center

Other organizations : Sage Bionetworks

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Nf1 knockout, 8
Nf1 flox, 9
Hoxb7-Cre, 47
Lats1 flox, 48
Lats2 flox, 48
ROSA26-YFP, 49
ROSA26-lacZ
Athymic nude mice (Foxn1^(-/-))

dependent variables

Measured outcomes

control variables

All mice were housed in the Animal Care Facility at The University of Texas Southwestern Medical Center (Dallas, TX)
All procedures were approved by Institutional Animal Care and Use Committee at University of Texas Southwestern Medical Center and conformed to NIH guidelines
Human subjects and cNF sample collection and use were approved by Institutional Review Board at University of Texas Southwestern Medical Center and conformed to NIH guidelines
Written informed consent was obtained from patients

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