The following antibodies were used for Western blot, immunofluorescence and PLA analyses: rabbit anti-BAG3 LP11 antibody (1:10000) was developed against a complete recombinant human BAG3 fused with glutathione S transferase (LP11; 1:10000 ) [16 (link)]; rabbit anti-HSPB8 against a C-terminal peptide (NELPQDSQEVTCT; 1:1000) [22 (link)]; anti-p62 SQSTM1 (sc-28359; 1:200) and anti-GFP (sc-9996, 1:1000; Santa Cruz); anti-GFP (632593, 1:500; Clontech); anti-HDAC6 (#7612; 1:1000) and anti-lysine-acetylated (ac-K-100, #9814, 1:1000; Cell Signaling Technologies); anti-vinculin (V9131; 1:5000), anti-Flag M2 (F3165, 1/200) and anti-α-tubulin mouse (T5168, 1:10000; Sigma-Aldrich); anti-cortactin (ab33333, 1:1000) and anti-α-tubulin rabbit (ab18251, 1:1000; Abcam); anti-acetyl-cortactin K309 (ABT1378, 1:1000); anti-cyclin B1 (ac-245, 1:1000) and anti-GAPDH Clone 6C5 (Fitzgerald, #10R-G109a, 1:10000) were from Millipore.
The Alexa-Fluor® 488 Phalloidin (A12379, 1/50) comes from Molecular Probes/Thermo Fisher. Tubacin (S2239) was from Selleckchem; bisbenzimide H 33,342 (Hoechst, B2261), thymidine (T9250), fibronectin (F1141-5 mg) and CK666 (SML0006) were from Sigma-Aldrich.
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