HPLC and GC/MS Analytical Workflow

High-performance liquid chromatography (HPLC 1260 Infinity II, Agilent, Santa Clara, CA, USA) analysis was carried out as described in [39 (link)] using isocratic separation with acetonitrile (20%) and water with 5mM ammonium formate and 0.1% formic acid (80%) at a constant flow rate of 1.5 mL/min. Gas chromatograph with mass selective detector (GC/MS 8860 GC/5977BMSD, Agilent, Santa Clara, CA) analysis was carried out also as described in [39 (link)].

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Anis O., Vinayaka A.C., Shalev N., Namdar D., Nadarajan S., Anil S.M., Cohen O., Belausov E., Ramon J., Mayzlish Gati E, & Koltai H. (2021). Cannabis-Derived Compounds Cannabichromene and Δ9-Tetrahydrocannabinol Interact and Exhibit Cytotoxic Activity against Urothelial Cell Carcinoma Correlated with Inhibition of Cell Migration and Cytoskeleton Organization. Molecules, 26(2), 465.

Publication 2021

HPLC 1260 Infinity II 8860 GC/5977BMSD

Acetonitrile Ammonium formate Formic acid Gas chromatograph Gc ms Hplc

Corresponding Organization : Agricultural Research Organization

Other organizations : Sheba Medical Center

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Variable analysis

independent variables

Acetonitrile concentration (20%)
Water with 5mM ammonium formate and 0.1% formic acid concentration (80%)

dependent variables

Chromatographic separation (HPLC)
Compound identification and quantification (GC/MS)

control variables

Constant flow rate (1.5 mL/min)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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