Mouse Dorsal Root Ganglion Neuron Isolation
Corresponding Organization : Instituto de Neurociencias
Variable analysis
- Enzymatic treatment: incubation in 900 U/mL type XI collagenase and 5.46 U/mL dispase for 45 min at 37 °C in 5% CO2
- Calcium imaging of cells
- Immunocytochemistry (ICC) after 10 min fixation in 4% PFA
- Mice used for DRG neuron extraction
- Removal and dissection of 20-40 DRGs
- Washing of dissected DRGs in cold HBSS solution
- Mechanical dissociation of DRGs using fire-polished glass pipettes
- Cell suspension centrifugation and resuspension in culture medium
- Seeding of cells onto 6 mm diameter glass coverslips coated with 0.01% poly-L-lysine
- Incubation of cells for 24 hours before calcium imaging or fixation
- Positive control: None mentioned
- Negative control: None mentioned
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