pAECs were collected from WT pigs and from two different genetically-modified pigs provided by the National Swine Resource and Research Center (NSRRC, Columbia, MO), namely GTKO and GTKO/hCD55 pigs. pAECs were isolated from fresh aortas and cultured, as previously described14 (link). Human aortic endothelial cells (hAECs) were purchased from Lonza (Walkersville, MD), and cultured as previously described14 (link).
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Isolation and Culture of pAECs
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Corresponding Organization : Shenzhen Second People's Hospital
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Variable analysis
independent variables
- Genotype of pigs: WT, GTKO, and GTKO/hCD55
- Isolation and culture of pAECs
- Isolation and culture of hAECs
- Culture conditions for pAECs and hAECs as previously described in reference 14
- Positive control: Not specified
- Negative control: Not specified
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