Pain severity was scored using the UNESP-Botucatu scale, a unidimensional composite pain scale previously validated for cattle undergoing orchiectomy (30 (link)). The UNESP-Botucatu scale is reported in detail in Supplementary Table S1. Briefly, five behaviors were monitored by an ordinal scale, ranging from 0 (normal) to 2 (totally abnormal), and applied to each parameter. Sedation was subjectively scored with a simple 0–3 sedation scale (0, fully alert or walking; 1, mildly sedated; 2, moderately sedated; 3, asleep) to be accounted for in the analysis.
Free full text: Click here