Protocol detail

Bladder Cancer Cell Culture Protocol

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Human bladder transitional cell carcinoma T24 (ATCC® HTB4™), TCCSUP (ATCC® HTB5™), and non-tumorigenic human bladder epithelial cells HBlEpC (938-05a) (Cell Applications Inc.) were cultured in McCoy’s 5A and Eagle’s Minimum Essential Medium (Millipore-Sigma), respectively, with 1% penicillin–streptomycin containing 10% fetal bovine serum (Gibco, Life Technologies) at 37 °C, in a 5% CO₂atmosphere. It was demonstrated that in vitro models can adequately reproduce clinically relevant results and may be suitable to identify novel substances for the treatment of bladder cancer (Vallo et al. 2015 (link)).

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Whynot E.G., Tomko A.M, & Dupré D.J. (2023). Anticancer properties of cannabidiol and Δ9-tetrahydrocannabinol and synergistic effects with gemcitabine and cisplatin in bladder cancer cell lines. Journal of Cannabis Research, 5, 7.

Publication 2023

HTB4 HTB5 HBlEpC (938-05a) penicillin–streptomycin fetal bovine serum

Bladder Cancer of the bladder Cell Eagle Epithelial cells Fetal bovine serum Human Penicillin Streptomycin Transitional cell carcinoma Tumorigenic

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Variable analysis

independent variables

Cell lines used: Human bladder transitional cell carcinoma T24 (ATCC® HTB4™), TCCSUP (ATCC® HTB5™), and non-tumorigenic human bladder epithelial cells HBlEpC (938-05a) (Cell Applications Inc.)

dependent variables

It was demonstrated that in vitro models can adequately reproduce clinically relevant results and may be suitable to identify novel substances for the treatment of bladder cancer

control variables

Cell culture conditions: McCoy's 5A and Eagle's Minimum Essential Medium (Millipore-Sigma), respectively, with 1% penicillin–streptomycin containing 10% fetal bovine serum (Gibco, Life Technologies) at 37 °C, in a 5% CO2 atmosphere

controls

No positive or negative controls were explicitly mentioned in the provided information.

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