ATCC MM.1S cells were cultured for 24, 48, and 72 hr with BMS309403 (50 µM), SBFI-26 (50 µM), or combination before staining with 500 nM CellROX for 30 min or 5 µM MitoSOX for 10 min per Thermofisher Scientific protocol. Data acquisition was performed on a Miltenyi MACSquant flow cytometer and data analysis was performed using FlowJo analysis software (BD Life Sciences) with a minimum of 10,000 events collected and gated off forward and side scatter plots.
Other organizations :
Maine Medical Center, MaineHealth, Tufts University, University of Maine, Dana-Farber Cancer Institute, Harvard University, University of Vermont, University of New England
Combination of BMS309403 (50 µM) and SBFI-26 (50 µM)
dependent variables
Oxidative stress levels measured by CellROX (500 nM, 30 min)
Mitochondrial superoxide levels measured by MitoSOX (5 µM, 10 min)
control variables
ATCC MM.1S cells
Staining protocols (CellROX and MitoSOX) as per Thermo Fisher Scientific
Data acquisition on Miltenyi MACSquant flow cytometer
Data analysis using FlowJo software (BD Life Sciences)
Minimum of 10,000 events collected and gated off forward and side scatter plots
controls
No controls explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
