Murine Hepatocyte Isolation and Culture

Hepatocytes were isolated from the murine liver using the collagenase perfusion method^{8 (link)} and subjected to primary culture on a bovine type I collagen-coated dish (AGC TECHNO GLASS, Shizuoka, Japan). In some experiments, primary hepatocytes were cultured in dishes precoated with recombinant human Jagged-1 Fc-chimera protein (R&D Systems, Minneapolis, MN). Gamma secretase inhibitor IX (DAPT; Merck, Darmstadt, Germany) was diluted in dimethyl sulfoxide (Sigma-Aldrich) before use.

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Nakano Y., Nakao S., Sueoka M., Kasahara D., Tanno Y., Sumiyoshi H., Itoh T., Miyajima A., Hozumi K, & Inagaki Y. (2022). Two distinct Notch signals, Delta-like 4/Notch1 and Jagged-1/Notch2, antagonistically regulate chemical hepatocarcinogenesis in mice. Communications Biology, 5, 85.

Publication 2022

recombinant human Jagged-1 Fc-chimera protein dimethyl sulfoxide

Bovine Chimera Collagenase Dapt Dimethyl sulfoxide Dishes Gamma secretase Hepatocytes Human Human jagged protein Liver Murine Perfusion Recombinant chimera protein Type i collagen

Corresponding Organization :

Other organizations : Tokai University, The University of Tokyo

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Variable analysis

independent variables

Culture of primary hepatocytes in dishes precoated with recombinant human Jagged-1 Fc-chimera protein
Treatment with gamma secretase inhibitor IX (DAPT)

dependent variables

Not explicitly mentioned

control variables

Culture of primary hepatocytes on a bovine type I collagen-coated dish

controls

Positive control: Primary hepatocytes cultured on bovine type I collagen-coated dish
Negative control: Not explicitly mentioned

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