Chitosan Immunomodulatory Effects Evaluation

Acetic acid, concanavalin A (Con A), dimethyl sulfoxide (DMSO), lipopolysaccharide (LPS) and YAC-1 cells were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). RPMI-1640 medium, fetal bovine serum (FBS), L-glutamine and antibiotics (penicillin-streptomycin) were purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Antibodies against CD3, CD19, CD11b and Mac-3 were obtained from BD Biosciences (San Jose, CA, USA). Chitosan powder with molecular weight of ~86,000 kDa (Koyo Chemical Co., Ltd, Osaka, Japan) was obtained from the National Taiwan University College of Medicine Animal Medicine Center (Taipei, Taiwan, Republic of China). It was dissolved in Acetic acid, and 5 and 20 mg/kg solutions were separately suspended in 0.2 ml Acetic acid at room temperature for 1 h before use (20 (link)).

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Yeh M.Y., Shih Y.L., Chung H.Y., Chou J., Lu H.F., Liu C.H., Liu J.Y., Huang W.W., Peng S.F., Wu L.Y, & Chung J.G. (2017). Chitosan promotes immune responses, ameliorating total mature white blood cell numbers, but increases glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, and ameliorates lactate dehydrogenase levels in leukemia mice in vivo. Molecular Medicine Reports, 16(3), 2483-2490.

Publication 2017

Acetic acid lipopolysaccharide (LPS) YAC-1 cells RPMI-1640 medium antibiotics (penicillin-streptomycin) CD11b Mac-3

Acetic acid Animal Antibiotics Antibodies Cd11b Cells Chitosan Concanavalin a Dimethyl sulfoxide Fetal bovine serum L glutamine Lipopolysaccharide Mac 3 Penicillin Powder Streptomycin

Corresponding Organization :

Other organizations : Cheng Hsin General Hospital, Fu Jen Catholic University, Kang-Ning Junior College of Medical Care and Management, Chia Nan University of Pharmacy and Science, China Medical University, I-Shou University

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Variable analysis

independent variables

Acetic acid
Chitosan powder with molecular weight of ~86,000 kDa (5 mg/kg and 20 mg/kg solutions)

dependent variables

Unspecified

control variables

RPMI-1640 medium
Fetal bovine serum (FBS)
L-glutamine
Antibiotics (penicillin-streptomycin)
Concanavalin A (Con A)
Dimethyl sulfoxide (DMSO)
Lipopolysaccharide (LPS)
YAC-1 cells
Antibodies against CD3, CD19, CD11b and Mac-3

positive controls

Concanavalin A (Con A)
Lipopolysaccharide (LPS)

negative controls

Dimethyl sulfoxide (DMSO)

Annotations

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