All experiments followed the ARVO and European Union guidelines for the use of animals in research and were approved by the Ethical and Animal Studies Committee (University of Murcia [UM]). Adult female albino Swiss mice (25–30 g) were obtained from Charles River Laboratories (L'Arbresle, France) and housed at the UM animal facilities in temperature and light controlled rooms (12-hour light/dark cycle) with food and water ad libitum. For surgical or animal manipulations, mice were anaesthetized with an intraperitoneal (ip) injection of ketamine (70 mg/kg Ketolar; Pfizer, Alcobendas, Madrid, Spain) and xylazine (10 mg/kg Rompun; Bayer, Kiel, Germany). Topical ointment (Tobrex; Alcon-Cusí, S.A., El Masnou, Barcelona, Spain) was applied during recovery to prevent corneal desiccation. Mice were euthanized with pentobarbital (Dolethal, Vetoquinol; Especialidades Veterinarias, S.A., Alcobendas, Madrid, Spain). In the present experiments we have used six naïve untouched mice, and 110 subjected to LIP; their left eyes were used as experimental while the right eyes served as controls. Because our previous studies characterizing the cone population of adult mice were done in female mice,55 (link) for comparison we have used only female mice in the present study.