Antibody Reagents for Golgi Apparatus Studies

All reagents were from Sigma-Aldrich or Merck Millipore, unless stated otherwise. Antibodies used in this study were against the following proteins: GMAP-210 (rabbit polyclonal; Sigma), GMAP-210 (mouse monoclonal; BD Biosciences), GM130 (mouse monoclonal; BD Biosciences), Myc (mouse monoclonal, 9B11; Cell Signaling Technology), Myc (rabbit polyclonal; Abcam), ERGIC53 (mouse monoclonal; Enzo Life Sciences), LAMP-1 (mouse monoclonal, H4A3; Developmental Studies Hybridoma Bank), GalNAcT2 (mouse monoclonal, UH-4; gift from Henrik Clausen), TfR (rabbit polyclonal; Millipore), GRASP65 (sheep; gift from Jon Lane), β-COP (mouse monoclonal, mAD; gift from Thomas Kreis), mannosidase II (rabbit polyclonal; Chemicon), and α-tubulin (mouse monoclonal; gift from Keith Gull). Mouse anti-p115 (4H1), rabbit anti-GM130, sheep anti-golgin-84, and sheep anti-GFP were described previously (Choudhury et al., 2009 (link); Diao et al., 2003 (link)). Sheep anti-GMAP-210 was raised against maltose binding protein fused to amino acids 1–375 of human GMAP-210 and affinity purified against the fusion protein. Fluorophore–conjugated secondary antibodies for microscopy and Western blotting were purchased from Molecular Probes and LI-COR Biosciences, respectively.