All experiments were carried out in accordance with the UBC Animal Care Committee and Canadian Council on Animal Care. Male non-diabetic 8–14 week old NSG mice (005557 NOD.Cg-Prkdc Il2rg/SzJ) were maintained on a 12-h light/dark cycle with ad libitum access to irradiated standard chow diet (#2919) and regular xGVHD monitoring19 . Blood glucose was measured in saphenous vein samples using a OneTouch Verio glucometer (LifeScan) or in intraperitoneal glucose-stimulated insulin secretion tests (ipGSIS), in which mice were fasted for 6 hours then challenged with 2 g/kg glucose. Human C-peptide and engrafted cell phenotype were measured weekly.
Protocol detail
Diabetic Mouse Glucose Monitoring
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Animal
Animal care committee
Blood glucose
C peptide
Cell
Diet
Glucose
Human
Il2rg
Insulin secretion
Male
Mice
Phenotype
Prkdc
Saphenous vein
Corresponding Organization : University of British Columbia
Other organizations : University of Alberta
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Variable analysis
independent variables
- Mouse genotype (NSG mice)
- Blood glucose levels
- Intraperitoneal glucose-stimulated insulin secretion (ipGSIS)
- Human C-peptide levels
- Engrafted cell phenotype
- Age of mice (8-14 weeks)
- Sex of mice (male)
- Housing conditions (12-h light/dark cycle, ad libitum access to irradiated standard chow diet)
- Regular xGVHD monitoring
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