iNKT cells were expanded from third-party PBMC with some minor modifications as previously described (Online Supplementary Appendix).13 (link) Culture-expanded iNKT cells were purified with antiiNKT MicroBeads (Miltenyi Biotech, Bergisch Gladbach, Germany). Alternatively, iNKT cells were stained with DAPI (4',6- diamidino-2-phenylindole, Merck, Darmstadt, Germany), anti- CD3, anti-CD4, anti-CD8 antibodies and PBS57-loaded CD1d tetramer allowing for enrichment of iNKT cells and their different subsets by fluorescence-activated cell sorting (FACS).
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Expansion and Purification of iNKT Cells
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Other organizations : University of Tübingen, Institute of Biochemistry
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- Expansion of iNKT cells from third-party PBMC with some minor modifications
- Purification of culture-expanded iNKT cells with anti-iNKT MicroBeads
- Enrichment of iNKT cells and their different subsets by fluorescence-activated cell sorting (FACS)
- The expansion of iNKT cells from third-party PBMC was performed as previously described (
Online Supplementary Appendix )
- No positive or negative controls were explicitly mentioned in the provided information.
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