Generation of Wnt1-Cre;Kdm6b-deficient Mice

To generate Wnt1^Cre;Kdm6b^fl/fl mice, we crossed Wnt1^Cre;Kdm6b^fl/+ mice with Kdm6b^fl/fl mice (Zhao et al., 2008 (link); Manna et al., 2015 (link)). Reporter mice used in this study were tdTomato conditional reporter (JAX#007905) (Madisen et al., 2010 (link)). Ezh2^fl/fl and Trp53^fl/fl mice were purchased from Jackson Laboratory (JAX#022616, #008462) (Marino et al., 2000 (link); Shen et al., 2008 (link)). Genotyping was carried out as previously described (Zhao et al., 2008 (link)). Briefly, tail samples were lysed by using DirectPCR tail solution (Viagen 102T) with overnight incubation at 55°C. After heat inactivation at 85°C for 1 hr, PCR-based genotyping (GoTaq Green MasterMix, Promega, and C1000 Touch Cycler, Bio-Rad) was used to detect the genes. All mouse studies were conducted with protocols approved by the Department of Animal Resources and the Institutional Animal Care and Use Committee (IACUC) of the University of Southern California (Protocols 9320 and 20299).