Mosquito Trapping Protocol with HDNs

During catches performed with HDNs one adult occupied one trap and according to the different experiments collected mosquitoes for six or eight hours. Participants rested on a metal-framed bed with fabric inlay (20 cm high x 200 cm long x 70 cm wide) and were fully protected from mosquitoes by a small blue polyester bed net (97 cm high x 200 cm long x 100 cm wide, mesh size 1.5 mm) which was not treated with any insecticide and which hung over the bed to the ground. A larger untreated bed net (100 cm high x 250 cm long x 150 cm wide, mesh size 1.5 mm) which was also not treated with insecticide was hung over the smaller net and was raised 30 cm above the ground[1 , 5 ]. Mosquitoes were caught in the ±20 cm gap between the two nets. Both nets were protected from the elements by plastic-sheeting roofs (Fig 1A). For 10 minutes of every hour participants raised the bottom of the inner net and aspirated mosquitoes caught between the nets into paper-cups. Mosquito catches of each hour were aspirated into different paper cups. When participants were not collecting mosquitoes, they rested inside the inner net. Participants had access to a stopwatch to monitor the time. Every collection day two field supervisors verified the participants conducted the collections as instructed.