We previously inserted dPDPN with an N-terminal PA tag (GVAMPGAEDDVV)(19 (link)) and a C-terminal RAP tag (DMVNPGLEDRIE)(20 (link))-MAP tag (GDGMVPPGIEDK)(21 (link)) (PA-dPDPN-RAP-MAP) in a pCAG-Ble vector (FUJIFILM Wako Pure Chemical Corporation).(11 (link)) CHO-K1 cells (American Type Culture Collection, Manassas, VA) were transfected with pCAG-Ble/PA-dPDPN-RAP-MAP using Gene Pulser Xcell electroporation system (Bio-Rad Laboratories, Inc., Berkeley, CA) for developing CHO/dPDPN.
CHO/dPDPN and CHO-K1 cells were cultured using RPMI-1640 medium (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Inc.). Antibiotics, such as 100 U/mL penicillin, 100 μg/mL streptomycin, and 25 μg/mL amphotericin B (Nacalai Tesque, Inc.) were added into the medium. Cells were cultivated at 37°C in a humidified atmosphere of 5% CO2 and 95% air.
CHO/dPDPN and CHO-K1 cells were cultured using RPMI-1640 medium (Nacalai Tesque, Inc., Kyoto, Japan) supplemented with 10% heat-inactivated fetal bovine serum (Thermo Fisher Scientific, Inc.). Antibiotics, such as 100 U/mL penicillin, 100 μg/mL streptomycin, and 25 μg/mL amphotericin B (Nacalai Tesque, Inc.) were added into the medium. Cells were cultivated at 37°C in a humidified atmosphere of 5% CO2 and 95% air.
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