Optical density was collected using a Tecan Infinite F200 for 96-sample plates. Each well/sample was measured at five different positions and the median value was used as the OD measure. A background level was measured in each plate and was subtracted from the measured OD.
Growth assays were conducted using a Tecan Freedom Evo 2000 liquid handling station. The 200 μl 96 sample plate was incubated at 30°C, for >24 h with an automatic scheduled OD measurement in the Tecan Infinite F200 executed every hour (65 (link)).
A log-linear fit was applied to each consecutive set of 10 data points, and the minimal doubling time was determined by the fit with the highest slope among the fits that passed the 5% significance threshold (test for linear fit, Bonferroni-corrected).
Growth assays were conducted using a Tecan Freedom Evo 2000 liquid handling station. The 200 μl 96 sample plate was incubated at 30°C, for >24 h with an automatic scheduled OD measurement in the Tecan Infinite F200 executed every hour (65 (link)).
A log-linear fit was applied to each consecutive set of 10 data points, and the minimal doubling time was determined by the fit with the highest slope among the fits that passed the 5% significance threshold (test for linear fit, Bonferroni-corrected).
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