Quantitative Northern Blot Analysis
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Institute of Biochemistry and Biophysics, Polish Academy of Sciences
Other organizations : International Institute of Molecular and Cell Biology, University of Warsaw
Variable analysis
- RNA isolation method (use of TRI Reagent according to manufacturer's instructions)
- RNA expression levels (quantified using Northern blot)
- Agarose gel electrophoresis and capillary transfer protocols
- Probe labeling method (DecaLabel DNA Labeling Kit with [α-32P] dATP)
- Probe generation method (PCR products with S6 or T7 promoter sequence)
- Phosphor screen exposure and scanning (Typhoon FLA 9000 scanner)
- Quantification software (Multi Gauge V3.0)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!