Murine Mesodermal Endothelial Progenitor Cell Differentiation

The murine mesodermal endothelial progenitor cell line T17b was cultured and differentiated according to established protocols [24 (link),30 (link)]. Briefly, T17b EPCs were seeded onto cell culture flasks coated with bovine skin gelatin type B (Sigma-Aldrich, Schnelldorf, Germany). For cell cultivation, high glucose DMEM GlutaMAX^® (Gibco/Life Technologies, Carlsbad, CA, USA) was used containing 20% fetal calf serum (Biochrom), 100 U/mL penicillin (Biochrom), 100 µg/mL streptomycin (Biochrom), 1 mM nonessential amino acids (Gibco), 2 mM HEPES buffer pH 7.5 (Gibco) and 0.1 mM 2-mercaptoethanol (Gibco). By supplementing 0.5 mM dibutyryl cyclic AMP and 1 µM all-trans retinoic acid (Sigma-Aldrich), endothelial differentiation was induced.

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Steiner D., Reinhardt L., Fischer L., Popp V., Körner C., Geppert C.I., Bäuerle T., Horch R.E, & Arkudas A. (2022). Impact of Endothelial Progenitor Cells in the Vascularization of Osteogenic Scaffolds. Cells, 11(6), 926.

Publication 2022

bovine skin gelatin type B GlutaMAX fetal calf serum penicillin streptomycin nonessential amino acids HEPES buffer pH 7.5 2-mercaptoethanol dibutyryl cyclic AMP

All trans retinoic acid Amino acids Bovine Buffer Cell Cell culture Dibutyryl cyclic amp Endothelial Endothelial progenitor cell Epcs Fetal calf serum Gelatin Glucose Hepes Mercaptoethanol Mesodermal Murine Penicillin Skin Streptomycin

Corresponding Organization : Friedrich-Alexander-Universität Erlangen-Nürnberg

Other organizations : Comprehensive Cancer Center Erlangen

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Variable analysis

independent variables

Supplementing 0.5 mM dibutyryl cyclic AMP
Supplementing 1 µM all-trans retinoic acid

dependent variables

Endothelial differentiation of T17b EPCs

control variables

T17b EPCs seeded onto cell culture flasks coated with bovine skin gelatin type B
Culture medium: High glucose DMEM GlutaMAX containing 20% fetal calf serum, 100 U/mL penicillin, 100 µg/mL streptomycin, 1 mM nonessential amino acids, 2 mM HEPES buffer pH 7.5, and 0.1 mM 2-mercaptoethanol

controls

No additional controls were explicitly mentioned in the provided information.

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