Protocol detail

Characterization of Antibodies for Cell Signaling

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Rabbit polyclonal anti-MCDL and anti-MEIK have been described and characterized previously (Liu et al., 2013 (link); Guo et al., 2017 (link)). Rabbit polyclonal anti-NBCn1 was custom-made against an immunogen “ENAKVTRPNMSPEKPVSVTC” by Genscript (Nanjing, CN). Rabbit anti-NHE3 (cat#ab-95299) and mouse anti-α1 (cat#ab-7671) against the α1 subunit of Na⁺-K⁺ pump (aka Na⁺-K⁺-ATPase) were purchased from Abcam (HK, China). Mouse anti-actin was purchased from Beyotime (Cat#AA128, Haimen, Jiangsu, China). HRP-conjugated secondary antibodies were from Beyotime. Alexa Fluor^® 488 AffiniPure goat anti-mouse (cat# 115-545-003) was from Jackson ImmunoResearch (West Grove, PA, United States). Dylight 549 goat anti-rabbit (cat#E032320-01) was from EarthOx (Millbrae, CA, United States).

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Wang J.L., Zhao L., Zhu J., Wang D.K., Ren M.J., Wang M., Liu Y., Boron W.F, & Chen L.M. (2019). Expression, Localization, and Effect of High Salt Intake on Electroneutral Na+/HCO3– Cotransporter NBCn2 in Rat Small Intestine: Implication in Intestinal NaCl Absorption. Frontiers in Physiology, 10, 1334.

Publication 2019

mouse anti-α1 Mouse anti-actin HRP-conjugated secondary antibodies Alexa Fluor® 488 AffiniPure goat anti-mouse Dylight 549 goat anti-rabbit

Actin Alexa fluor 488 Antibodies Goat Immunogen Mouse Na k atpase Rabbit Subunit

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Variable analysis

independent variables

Rabbit polyclonal anti-MCDL and anti-MEIK antibodies
Rabbit polyclonal anti-NBCn1 antibody
Rabbit anti-NHE3 antibody
Mouse anti-α1 (Na+/K+-ATPase) antibody
Mouse anti-actin antibody

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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