Immunostaining Protocol for Cell Visualization
Protocol cited in 1 other protocol
Variable analysis
- Immunostaining protocol
- Expression/localization of CD68, IBA1, iNOS, GFAP, MBP, and MAP-2
- Cell density (200-300 cells/mm^2)
- Fixation with 4% paraformaldehyde for 15 min
- Treatment with cold methanol (-20 °C) for 5 min
- Blocking with 2% BSA in PBST for 30 min
- Primary antibody incubation (1% BSA in PBST)
- Secondary antibody incubation (Cy2, Cy5, DAPI)
- Mounting in Fluoromount (Sigma)
- Imaging using Olympus BX41 fluorescent microscope and Hamamatsu ORCA-03G camera
- Not specified
- Not specified
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