Cell Viability Assessment using CCK-8

Cells were plated in 96-well plates at a density of 1 × 10³ cells per well, following standard protocols (45 (link), 46 (link)). Following that, the plates were incubated in darkness at 37°C for 2 hours with CCK-8 labeling reagent (A311-01, Vazyme). The assessment of cell viability was carried out by measuring the absorbance at 450 nm using an enzyme-linked spectrophotometer (A33978, Thermo) at time intervals of 0, 24, 48, 72, and 96 hours.

Free full text: Click here

Cai H.B., Zhao M.Y., Li X.H., Li Y.Q., Yu T.H., Wang C.Z., Wang L.N., Xu W.Y., Liang B., Cai Y.P., Zhang F, & Hong W.M. (2024). Single cell sequencing revealed the mechanism of CRYAB in glioma and its diagnostic and prognostic value. Frontiers in Immunology, 14, 1336187.

Publication 2023

A311-01 A33978

Corresponding Organization : Anhui Medical University

Other organizations : Shandong University of Traditional Chinese Medicine

Top 5 similar protocols

Variable analysis

independent variables

Incubation time (0, 24, 48, 72, 96 hours)

dependent variables

Cell viability (measured by absorbance at 450 nm)

control variables

Cell plating density (1 × 10^3 cells per well)
Incubation temperature (37°C)
Incubation duration (2 hours)
Reagent used (CCK-8 labeling reagent)
Measurement instrument (enzyme-linked spectrophotometer)

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!