Raw 264.7 cells were maintained in RPMI 1640 supplemented with 10% FBS (Fetal Bovine Serum, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% antibiotic-anti-myotic (Gibco, Grand Island, NY, USA) at 37 °C, 5% CO2 in humidified air. Influenza A (A/PR/8/34) and GFP-tagged A/PR/8/34 (A/PR/8/34-GFP) viruses were used in the previous studies [15 (link)].
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