Rat Basophilic Leukemia Cell Culture

All experiments were performed with rat basophilic leukaemia cells (RBL-703/21-derived NFATp-DsRed-Express2) generated at The University of Nottingham^{16 (link)}. These are available from the authors for non-commercial purposes on the basis of a material transfer agreement (MTA). Cells were grown in Minimum Essential Medium Eagle’s (EMEM) cell culture medium supplemented with 10% v/v heat-inactivated foetal bovine serum (Gibco, UK), 100 U/mL penicillin, 100 μg/mL streptomycin and 2 mM L-glutamine (Merck, UK) in a 37 °C/5% CO₂ humidified cell incubator. To maintain stable expression of FcεRIα_H, cells were cultured with 1 mg/mL G418 sulphate (Thermo Fisher Scientific, UK) and 20 μg/mL Blasticidin S HCL (InvivoGen, USA) for selection of NFATp-DsRed-Express2 reporter gene expression.