In vivo and ex vivo bioluminescence imaging

In vivo bioluminescence was detected as previously described using an optical imaging device (Ami-X, Spectral Instruments Imaging) [17 (link)]. To detect in vivo bioluminescence signal, mice were i.p. injected with D-luciferin (150 mg kg⁻¹, Goldbio, St. Louis, MO, USA). Aura Software (Spectral Instruments Imaging, Version 2.2.1.1) was used for quantification. Also, ex vivo imaging, tissues were collected from the CIDEA reporter mice treated with D-luciferin (150 mg kg⁻¹, Goldbio, St. Louis, MO, USA). During imaging, the isolated tissues were maintained in 12 well plates containing D-luciferin (300 μg/ml).

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Kim K., Im H., Son Y., Kim M., Tripathi S.K., Jeong L.S, & Lee Y.H. (2022). Anti-obesity effects of the dual-active adenosine A2A/A3 receptor-ligand LJ-4378. International Journal of Obesity (2005), 46(12), 2128-2136.

Publication 2022

Ami-X D-luciferin Aura Software

Device Luciferin Mice Tissues

Corresponding Organization :

Other organizations : Seoul National University

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Variable analysis

independent variables

D-luciferin injection (150 mg kg^-1)

dependent variables

In vivo bioluminescence signal
Ex vivo bioluminescence signal of isolated tissues

control variables

Use of Aura Software (Spectral Instruments Imaging, Version 2.2.1.1) for quantification
Maintenance of isolated tissues in 12 well plates containing D-luciferin (300 μg/ml) during ex vivo imaging

controls

Positive control: Mice treated with D-luciferin (150 mg kg^-1)
Negative control: Not explicitly mentioned

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