Escherichia coli DH5α
was used for cloning following standard recombinant DNA techniques. E. coli BL21(DE3) was used for expression of protein. Aspergillus oryzae NSAR1 (niaD–, sC–, ΔargB, adeA–) and A. oryzae NSPlD1 (niaD–, sC–, ΔpryG, ΔligD) were used as the host for fungal expression. Discosia sp. batta1 was used for genomic DNA preparation
and draft genome sequencing. Pestalotiopsis fici MAFF
237190, P. microspora NBRC 30316, and Aspergillus
candidus NBRC 8816 were used for genomic DNA preparation.
was used for cloning following standard recombinant DNA techniques. E. coli BL21(DE3) was used for expression of protein. Aspergillus oryzae NSAR1 (niaD–, sC–, ΔargB, adeA–) and A. oryzae NSPlD1 (niaD–, sC–, ΔpryG, ΔligD) were used as the host for fungal expression. Discosia sp. batta1 was used for genomic DNA preparation
and draft genome sequencing. Pestalotiopsis fici MAFF
237190, P. microspora NBRC 30316, and Aspergillus
candidus NBRC 8816 were used for genomic DNA preparation.
